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Article Open Access

MicroRNA‑936 inhibits the malignant phenotype of retinoblastoma by directly targeting HDAC9 and deactivating the PI3K/AKT pathway

Retraction in: /10.3892/or.2022.8389
  • Authors:
    • Lishuai Xu
    • Weidong Li
    • Qian Shi
    • Minfeng Wang
    • Heng Li
    • Xiaoli Yang
    • Junjun Zhang
  • View Affiliations / Copyright

    Affiliations: Department of Ophthalmology and Optometry, North Sichuan Medical College, Nanchong, Sichuan 637000, P.R. China, Department of Cardiology, Affiliated Hospital of North Sichuan Medical College, Nanchong, Sichuan 637100, P.R. China, Department of Ophthalmology, Yixing Eye Hospital, Yixing, Jiangsu 214200, P.R. China, Department of Ophthalmology, Suining Central Hospital, Suining, Sichuan 637000, P.R. China, Department of Ophthalmology, West China Hospital, Sichuan University, Chengdu, Sichuan 610041, P.R. China
    Copyright: © Xu et al. This is an open access article distributed under the terms of Creative Commons Attribution License [CC BY_NC 4.0].
  • Pages: 635-645
    |
    Published online on: January 9, 2020
       https://doi.org/10.3892/or.2020.7456
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Abstract

MicroRNA‑936 (miR‑936) has been reported to play important roles in the progression of non‑small cell lung cancer and glioma. However, the expression and functions of miR‑936 in retinoblastoma (RB) remain elusive and need to be further elucidated. Herein, the aims were to measure miR‑936 expression in RB, identify the functional importance of miR‑936 in the oncogenicity of RB, and investigate the underlying molecular mechanisms. Reverse‑transcription quantitative PCR was carried out to determine miR‑936 expression in RB tissues and cell lines. Cell proliferation, colony formation, apoptosis, migration, and invasion in vitro and tumor growth in vivo were examined respectively by Cell Counting Kit‑8, colony formation, flow cytometric, and Transwell migration and invasion assays and a subcutaneous heterotopic xenograft experiment. The potential target of miR‑936 was predicted by bioinformatic analysis and was subsequently validated by luciferase reporter assay, reverse‑transcription quantitative PCR, and western blotting. miR‑936 expression was weak in both RB tissues and cell lines and was correlated with differentiation, lymph node metastasis and TNM staging in RB. RB cell proliferation, colony formation, migration, and invasion in vitro and tumor growth in vivo were attenuated by exogenous miR‑936, whereas apoptosis was enhanced by miR‑936 overexpression. Further molecular investigation identified histone deacetylase 9 (HDAC9) as a direct target gene of miR‑936 in RB cells. HDAC9 depletion had effects similar to those of miR‑936 overexpression in RB cells. Recovery of HDAC9 expression counteracted the tumor‑suppressive action of miR‑936 on the oncogenicity of RB cells. Ectopic miR‑936 expression deactivated the PI3K/AKT pathway in RB cells in vitro and in vivo by decreasing HDAC9 expression. Downregulated miR‑936 is related to poor prognosis in RB, and its upregulation inhibits RB aggressiveness via direct targeting of HDAC9 mRNA and thereby inactivation of the PI3K/AKT pathway.
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1 

Dimaras H, Kimani K, Dimba EA, Gronsdahl P, White A, Chan HS and Gallie BL: Retinoblastoma. Lancet. 379:1436–1446. 2012. View Article : Google Scholar : PubMed/NCBI

2 

Kivelä T: The epidemiological challenge of the most frequent eye cancer: Retinoblastoma, an issue of birth and death. Br J Ophthalmol. 93:1129–1131. 2009. View Article : Google Scholar : PubMed/NCBI

3 

He MY, An Y, Gao YJ, Qian XW, Li G and Qian J: Screening of RB1 gene mutations in Chinese patients with retinoblastoma and preliminary exploration of genotype-phenotype correlations. Mol Vis. 20:545–552. 2014.PubMed/NCBI

4 

Balmer A, Zografos L and Munier F: Diagnosis and current management of retinoblastoma. Oncogene. 25:5341–5349. 2006. View Article : Google Scholar : PubMed/NCBI

5 

Tian T, Ji XD, Zhang Q, Peng J and Zhao PQ: A delayed diagnosis of unsuspected retinoblastoma in an in vitro fertilisation infant with retinopathy of prematurity. Int J Ophthalmol. 9:1361–1363. 2016.PubMed/NCBI

6 

Abramson DH, Shields CL, Munier FL and Chantada GL: Treatment of retinoblastoma in 2015: Agreement and disagreement. JAMA Ophthalmol. 133:1341–1347. 2015. View Article : Google Scholar : PubMed/NCBI

7 

Correa-Acosta A, González-Alviar ME and Gaviria-Bravo ML: Retinoblastoma and optic nerve enhancement in a brain magnetic resonance scan: Is it always a metastasis? Arch Soc Esp Oftalmol. 93:251–254. 2018.(In English, Spanish). View Article : Google Scholar : PubMed/NCBI

8 

Lytle JR, Yario TA and Steitz JA: Target mRNAs are repressed as efficiently by microRNA-binding sites in the 5′ UTR as in the 3′ UTR. Proc Natl Acad Sci USA. 104:9667–9672. 2007. View Article : Google Scholar : PubMed/NCBI

9 

Guarnieri DJ and DiLeone RJ: MicroRNAs: A new class of gene regulators. Ann Med. 40:197–208. 2008. View Article : Google Scholar : PubMed/NCBI

10 

Delsin LEA, Salomao KB, Pezuk JA and Brassesco MS: Expression profiles and prognostic value of miRNAs in retinoblastoma. J Cancer Res Clin Oncol. 145:1–10. 2019. View Article : Google Scholar : PubMed/NCBI

11 

Li W, Wang J, Zhang D, Zhang X, Xu J and Zhao L: MicroRNA-98 targets HMGA2 to inhibit the development of retinoblastoma through mediating Wnt/β-catenin pathway. Cancer Biomark. 25:79–88. 2019. View Article : Google Scholar : PubMed/NCBI

12 

Wu S, Han M and Zhang C: Overexpression of microRNA-186 inhibits angiogenesis in retinoblastoma via the Hedgehog signaling pathway by targeting ATAD2. J Cell Physiol. 234:19059–19072. 2019.PubMed/NCBI

13 

Wang S, Du S, Lv Y, Zhang F and Wang W: MicroRNA-665 inhibits the oncogenicity of retinoblastoma by directly targeting high-mobility group box 1 and inactivating the Wnt/β-catenin pathway. Cancer Manag Res. 11:3111–3123. 2019. View Article : Google Scholar : PubMed/NCBI

14 

Cao Y, Xia F, Wang P and Gao M: MicroRNA-93-5p promotes the progression of human retinoblastoma by regulating the PTEN/PI3K/AKT signaling pathway. Mol Med Rep. 18:5807–5814. 2018.PubMed/NCBI

15 

Bu W, Wang Y and Min X: MicroRNA-106b promotes the proliferation, migration and invasion of retinoblastoma cells by inhibiting the expression of ZBTB4 protein. Exp Ther Med. 16:4537–4545. 2018.PubMed/NCBI

16 

Zhou X and Tao H: Overexpression of microRNA-936 suppresses non-small cell lung cancer cell proliferation and invasion via targeting E2F2. Exp Ther Med. 16:2696–2702. 2018.PubMed/NCBI

17 

Wang D, Zhi T, Xu X, Bao Z, Fan L, Li Z, Ji J and Liu N: MicroRNA-936 induces cell cycle arrest and inhibits glioma cell proliferation by targeting CKS1. Am J Cancer Res. 7:2131–2143. 2017.PubMed/NCBI

18 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods. 25:402–408. 2001. View Article : Google Scholar : PubMed/NCBI

19 

Yang R, Wu Y, Wang M, Sun Z, Zou J, Zhang Y and Cui H: HDAC9 promotes glioblastoma growth via TAZ-mediated EGFR pathway activation. Oncotarget. 6:7644–7656. 2015.PubMed/NCBI

20 

Li J, Zhang Y, Wang X and Zhao R: microRNA-497 overexpression decreases proliferation, migration and invasion of human retinoblastoma cells via targeting vascular endothelial growth factor A. Oncol Lett. 13:5021–5027. 2017. View Article : Google Scholar : PubMed/NCBI

21 

Liang Y, Chen X and Liang Z: MicroRNA-320 regulates autophagy in retinoblastoma by targeting hypoxia inducible factor-1α. Exp Ther Med. 14:2367–2372. 2017. View Article : Google Scholar : PubMed/NCBI

22 

Li J and You X: MicroRNA-758 inhibits malignant progression of retinoblastoma by directly targeting PAX6. Oncol Rep. 40:1777–1786. 2018.PubMed/NCBI

23 

Golabchi K, Soleimani-Jelodar R, Aghadoost N, Momeni F, Moridikia A, Nahand JS, Masoudifar A, Razmjoo H and Mirzaei H: MicroRNAs in retinoblastoma: Potential diagnostic and therapeutic biomarkers. J Cell Physiol. 233:3016–3023. 2018. View Article : Google Scholar : PubMed/NCBI

24 

Singh A, Patel P, Patel VK, Jain DK, Veerasamy R, Sharma PC and Rajak H: Histone deacetylase inhibitors for the treatment of colorectal cancer: Recent progress and future prospects. Curr Cancer Drug Targets. 17:456–466. 2017. View Article : Google Scholar : PubMed/NCBI

25 

Dokmanovic M and Marks PA: Prospects: Histone deacetylase inhibitors. J Cell Biochem. 96:293–304. 2005. View Article : Google Scholar : PubMed/NCBI

26 

Marks PA, Richon VM, Kelly WK, Chiao JH and Miller T: Histone deacetylase inhibitors: Development as cancer therapy. Novartis Found Symp. 259:269–281; discussion 281–288. 2004.PubMed/NCBI

27 

Zhang Y, Wu D, Xia F, Xian H, Zhu X, Cui H and Huang Z: Downregulation of HDAC9 inhibits cell proliferation and tumor formation by inducing cell cycle arrest in retinoblastoma. Biochem Biophys Res Commun. 473:600–606. 2016. View Article : Google Scholar : PubMed/NCBI

28 

Jin Q, He W, Chen L, Yang Y, Shi K and You Z: MicroRNA-101-3p inhibits proliferation in retinoblastoma cells by targeting EZH2 and HDAC9. Exp Ther Med. 16:1663–1670. 2018.PubMed/NCBI

29 

Li M, Tang Y, Wu L, Mo F, Wang X, Li H, Qi R, Zhang H, Srivastava A and Ling C: The hepatocyte-specific HNF4α/miR-122 pathway contributes to iron overload-mediated hepatic inflammation. Blood. 130:1041–1051. 2017. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Xu L, Li W, Shi Q, Wang M, Li H, Yang X and Zhang J: MicroRNA‑936 inhibits the malignant phenotype of retinoblastoma by directly targeting HDAC9 and deactivating the PI3K/AKT pathway Retraction in /10.3892/or.2022.8389. Oncol Rep 43: 635-645, 2020.
APA
Xu, L., Li, W., Shi, Q., Wang, M., Li, H., Yang, X., & Zhang, J. (2020). MicroRNA‑936 inhibits the malignant phenotype of retinoblastoma by directly targeting HDAC9 and deactivating the PI3K/AKT pathway Retraction in /10.3892/or.2022.8389. Oncology Reports, 43, 635-645. https://doi.org/10.3892/or.2020.7456
MLA
Xu, L., Li, W., Shi, Q., Wang, M., Li, H., Yang, X., Zhang, J."MicroRNA‑936 inhibits the malignant phenotype of retinoblastoma by directly targeting HDAC9 and deactivating the PI3K/AKT pathway Retraction in /10.3892/or.2022.8389". Oncology Reports 43.2 (2020): 635-645.
Chicago
Xu, L., Li, W., Shi, Q., Wang, M., Li, H., Yang, X., Zhang, J."MicroRNA‑936 inhibits the malignant phenotype of retinoblastoma by directly targeting HDAC9 and deactivating the PI3K/AKT pathway Retraction in /10.3892/or.2022.8389". Oncology Reports 43, no. 2 (2020): 635-645. https://doi.org/10.3892/or.2020.7456
Copy and paste a formatted citation
x
Spandidos Publications style
Xu L, Li W, Shi Q, Wang M, Li H, Yang X and Zhang J: MicroRNA‑936 inhibits the malignant phenotype of retinoblastoma by directly targeting HDAC9 and deactivating the PI3K/AKT pathway Retraction in /10.3892/or.2022.8389. Oncol Rep 43: 635-645, 2020.
APA
Xu, L., Li, W., Shi, Q., Wang, M., Li, H., Yang, X., & Zhang, J. (2020). MicroRNA‑936 inhibits the malignant phenotype of retinoblastoma by directly targeting HDAC9 and deactivating the PI3K/AKT pathway Retraction in /10.3892/or.2022.8389. Oncology Reports, 43, 635-645. https://doi.org/10.3892/or.2020.7456
MLA
Xu, L., Li, W., Shi, Q., Wang, M., Li, H., Yang, X., Zhang, J."MicroRNA‑936 inhibits the malignant phenotype of retinoblastoma by directly targeting HDAC9 and deactivating the PI3K/AKT pathway Retraction in /10.3892/or.2022.8389". Oncology Reports 43.2 (2020): 635-645.
Chicago
Xu, L., Li, W., Shi, Q., Wang, M., Li, H., Yang, X., Zhang, J."MicroRNA‑936 inhibits the malignant phenotype of retinoblastoma by directly targeting HDAC9 and deactivating the PI3K/AKT pathway Retraction in /10.3892/or.2022.8389". Oncology Reports 43, no. 2 (2020): 635-645. https://doi.org/10.3892/or.2020.7456
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