Open Access

Phlorizin from sweet tea inhibits the progress of esophageal cancer by antagonizing the JAK2/STAT3 signaling pathway

  • Authors:
    • Zhenxian Jia
    • Yuning Xie
    • Hongjiao Wu
    • Zhuo Wang
    • Ang Li
    • Ze Li
    • Zhenbang Yang
    • Zhi Zhang
    • Zhaobin Xing
    • Xuemei Zhang
  • View Affiliations

  • Published online on: May 21, 2021     https://doi.org/10.3892/or.2021.8088
  • Article Number: 137
  • Copyright: © Jia et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Phlorizin, an important member of the dihydrochalcone family, has been widely used as a Chinese Traditional Medicine for treatment of numerous diseases. The present study aimed to investigate the potential therapeutic effects of phlorizin on esophageal cancer. Phlorizin, extracted from sweet tea, was used to treat esophageal cancer cells. Cell proliferation, migration and invasion were determined using Cell Counting Kit‑8 and colony formation assays, and wound healing and Transwell assays, respectively. RNA sequencing and bioinformatics analysis was used to investigate the potential mechanism of phlorizin in the development of esophageal cancer. Fluorescent staining and flow cytometry was used to measure the level of apoptosis. The expression level of the proteins, P62/SQSTM1 and LC3 І/II, and the effect of phlorizin on the JAK2/STAT3 signaling pathway was detected using western blot analysis. The results demonstrated that phlorizin could inhibit cell proliferation, migration and invasion. Bioinformatics analysis showed that phlorizin might be involved in pleiotropic effects, such as the ‘JAK/STAT signaling pathway’ (hsa04630), ‘MAPK signaling pathway’(hsa04010) and ‘apoptosis’ (hsa04210). It was also confirmed that phlorizin promoted apoptosis and inhibited autophagy in the esophageal cancer cells. Notably, phlorizin might inhibit the proteins in the JAK/STAT signaling pathway, which would affect cancer cells. Taken together, the present data showed that phlorizin inhibited the progression of esophageal cancer by antagonizing the JAK2/STAT3 signaling pathway.
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July-2021
Volume 46 Issue 1

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Jia Z, Xie Y, Wu H, Wang Z, Li A, Li Z, Yang Z, Zhang Z, Xing Z, Zhang X, Zhang X, et al: Phlorizin from sweet tea inhibits the progress of esophageal cancer by antagonizing the JAK2/STAT3 signaling pathway. Oncol Rep 46: 137, 2021
APA
Jia, Z., Xie, Y., Wu, H., Wang, Z., Li, A., Li, Z. ... Zhang, X. (2021). Phlorizin from sweet tea inhibits the progress of esophageal cancer by antagonizing the JAK2/STAT3 signaling pathway. Oncology Reports, 46, 137. https://doi.org/10.3892/or.2021.8088
MLA
Jia, Z., Xie, Y., Wu, H., Wang, Z., Li, A., Li, Z., Yang, Z., Zhang, Z., Xing, Z., Zhang, X."Phlorizin from sweet tea inhibits the progress of esophageal cancer by antagonizing the JAK2/STAT3 signaling pathway". Oncology Reports 46.1 (2021): 137.
Chicago
Jia, Z., Xie, Y., Wu, H., Wang, Z., Li, A., Li, Z., Yang, Z., Zhang, Z., Xing, Z., Zhang, X."Phlorizin from sweet tea inhibits the progress of esophageal cancer by antagonizing the JAK2/STAT3 signaling pathway". Oncology Reports 46, no. 1 (2021): 137. https://doi.org/10.3892/or.2021.8088