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Article Open Access

Bioinformatics and experimental validation of an AURKA/TPX2 axis as a potential target in esophageal squamous cell carcinoma

  • Authors:
    • Ruijuan Du
    • Kai Li
    • Zijun Zhou
    • Yunlong Huang
    • Kelei Guo
    • Hui Zhang
    • Zhiguo Chen
    • Xulin Zhao
    • Li Han
    • Hua Bian
  • View Affiliations / Copyright

    Affiliations: Zhang Zhongjing School of Chinese Medicine, Nanyang Institute of Technology, Nanyang, Henan 473004, P.R. China, Oncology Department, Nanyang First People's Hospital, Nanyang, Henan 473004, P.R. China
    Copyright: © Du et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 116
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    Published online on: April 20, 2023
       https://doi.org/10.3892/or.2023.8553
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Abstract

Aurora kinase A (AURKA), a serine/threonine kinase that regulates mitotic processes, has garnered significant interest given its association with the development of several types of cancer. In the present study, it was shown that AURKA expression was significantly upregulated in esophageal squamous cell carcinoma (ESCC) and could serve as a diagnostic and prognostic indicator based on data obtained from The Cancer Genome Atlas (TCGA) and immunohistochemical analysis. In addition, AURKA was functionally associated with ESCC cell proliferation and colony formation in vitro and knockdown of AURKA inhibited ESCC tumor growth in vivo. Both bioinformatics analysis and pull‑down assays demonstrated that TPX2 interacted with AURKA, and their expression was correlated. AURKA cooperated with TPX2 to regulate ESCC progression via the PI3K/Akt pathway. Furthermore, AURKA or TPX2 expression levels were negatively associated with the infiltration of cytotoxic cells, CD8+ T cells and mast cells, but positively associated with Th2 cells. The present study provided a relatively comprehensive understanding of the oncogenic roles of AURKA in ESCC based on data obtained from TCGA combined with experimental analysis.
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Copy and paste a formatted citation
Spandidos Publications style
Du R, Li K, Zhou Z, Huang Y, Guo K, Zhang H, Chen Z, Zhao X, Han L, Bian H, Bian H, et al: Bioinformatics and experimental validation of an AURKA/TPX2 axis as a potential target in esophageal squamous cell carcinoma. Oncol Rep 49: 116, 2023.
APA
Du, R., Li, K., Zhou, Z., Huang, Y., Guo, K., Zhang, H. ... Bian, H. (2023). Bioinformatics and experimental validation of an AURKA/TPX2 axis as a potential target in esophageal squamous cell carcinoma. Oncology Reports, 49, 116. https://doi.org/10.3892/or.2023.8553
MLA
Du, R., Li, K., Zhou, Z., Huang, Y., Guo, K., Zhang, H., Chen, Z., Zhao, X., Han, L., Bian, H."Bioinformatics and experimental validation of an AURKA/TPX2 axis as a potential target in esophageal squamous cell carcinoma". Oncology Reports 49.6 (2023): 116.
Chicago
Du, R., Li, K., Zhou, Z., Huang, Y., Guo, K., Zhang, H., Chen, Z., Zhao, X., Han, L., Bian, H."Bioinformatics and experimental validation of an AURKA/TPX2 axis as a potential target in esophageal squamous cell carcinoma". Oncology Reports 49, no. 6 (2023): 116. https://doi.org/10.3892/or.2023.8553
Copy and paste a formatted citation
x
Spandidos Publications style
Du R, Li K, Zhou Z, Huang Y, Guo K, Zhang H, Chen Z, Zhao X, Han L, Bian H, Bian H, et al: Bioinformatics and experimental validation of an AURKA/TPX2 axis as a potential target in esophageal squamous cell carcinoma. Oncol Rep 49: 116, 2023.
APA
Du, R., Li, K., Zhou, Z., Huang, Y., Guo, K., Zhang, H. ... Bian, H. (2023). Bioinformatics and experimental validation of an AURKA/TPX2 axis as a potential target in esophageal squamous cell carcinoma. Oncology Reports, 49, 116. https://doi.org/10.3892/or.2023.8553
MLA
Du, R., Li, K., Zhou, Z., Huang, Y., Guo, K., Zhang, H., Chen, Z., Zhao, X., Han, L., Bian, H."Bioinformatics and experimental validation of an AURKA/TPX2 axis as a potential target in esophageal squamous cell carcinoma". Oncology Reports 49.6 (2023): 116.
Chicago
Du, R., Li, K., Zhou, Z., Huang, Y., Guo, K., Zhang, H., Chen, Z., Zhao, X., Han, L., Bian, H."Bioinformatics and experimental validation of an AURKA/TPX2 axis as a potential target in esophageal squamous cell carcinoma". Oncology Reports 49, no. 6 (2023): 116. https://doi.org/10.3892/or.2023.8553
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