[Corrigendum] CDCA5 promotes the progression of prostate cancer by affecting the ERK signalling pathway

Figure 3.

Knockdown of CDCA5 inhibits PCa cell proliferation and invasion in vitro. (A) Western blotting was used to detect the expression levels of CDCA5, Ki67 and cleaved/pro caspase-3 after CDCA5 knockdown in C4-2 and PC-3 cells. GAPDH was used as an internal control. (B) Flow cytometry was used to detect apoptosis in C4-2 and PC-3 cells after CDCA5 knockdown. The apoptotic rate (early apoptosis + late apoptosis) of C4-2 and PC-3 after knockdown CDCA5 was counted and plotted on a graph (**P<0.01 and ***P<0.001). The UL region indicates cell necrosis, the LL region indicates cell survival, the LR region indicates early cell apoptosis, and the UR region indicates late cell apoptosis. The LR region + UR region indicate total apoptosis. (C) An MTT assay was used to detect C4-2 and PC-3 cell growth after CDCA5 was knocked down. The absorbance value at a wavelength of 570 nm was detected (*P<0.05). (D) A colony formation assay was used to detect C4-2 and PC-3 cell growth after CDCA5 knockdown. (E) The number of colonies from D was counted and plotted (***P<0.001). (F) Transwell invasion assays detected the invasiveness of the prostate cancer cells after knockdown of CDCA5. (G) The number of invaded cells in F was counted and plotted on a graph (***P<0.001). CDCA5, cell division cycle-associated 5; PCa, prostate cancer; sh, short hairpin; CON, control; UL, upper left; LL, lower left; LR, lower right; UR, upper right..