Wnt/β‑catenin signaling is a novel therapeutic target for tumor suppressor CYLD‑silenced glioblastoma cells

  • Authors:
    • Ayumi Kanemaru
    • Yuki Ito
    • Michiko Yamaoka
    • Yuki Shirakawa
    • Kou Yonemaru
    • Shunsuke Miyake
    • Misaki Ando
    • Masako Ota
    • Takeshi Masuda
    • Akitake Mukasa
    • Jian-Dong Li
    • Hideyuki Saito
    • Takuichiro Hide
    • Hirofumi Jono
  • View Affiliations

  • Published online on: September 29, 2023     https://doi.org/10.3892/or.2023.8638
  • Article Number: 201
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Abstract

Tumor suppressor cylindromatosis (CYLD) dysfunction by its downregulation is significantly associated with poor prognosis in patients with glioblastoma (GBM), the most aggressive and malignant type of glioma. However, no effective treatment is currently available for patients with CYLD‑downregulated GBM. The aim of the present study was to identify the crucial cell signaling pathways and novel therapeutic targets for CYLD downregulation in GBM cells. CYLD knockdown in GBM cells induced GBM malignant characteristics, such as proliferation, metastasis, and GBM stem‑like cell (GSC) formation. Comprehensive proteomic analysis and RNA sequencing data from the tissues of patients with GBM revealed that Wnt/β‑catenin signaling was significantly activated by CYLD knockdown in patients with GBM. Furthermore, a Wnt/β‑catenin signaling inhibitor suppressed all CYLD knockdown‑induced malignant characteristics of GBM. Taken together, the results of the present study revealed that Wnt/β‑catenin signaling is responsible for CYLD silencing‑induced GBM malignancy; therefore, targeting Wnt/β‑catenin may be effective for the treatment of CYLD‑negative patients with GBM with poor prognosis.
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November-2023
Volume 50 Issue 5

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Kanemaru A, Ito Y, Yamaoka M, Shirakawa Y, Yonemaru K, Miyake S, Ando M, Ota M, Masuda T, Mukasa A, Mukasa A, et al: Wnt/β‑catenin signaling is a novel therapeutic target for tumor suppressor CYLD‑silenced glioblastoma cells. Oncol Rep 50: 201, 2023
APA
Kanemaru, A., Ito, Y., Yamaoka, M., Shirakawa, Y., Yonemaru, K., Miyake, S. ... Jono, H. (2023). Wnt/β‑catenin signaling is a novel therapeutic target for tumor suppressor CYLD‑silenced glioblastoma cells. Oncology Reports, 50, 201. https://doi.org/10.3892/or.2023.8638
MLA
Kanemaru, A., Ito, Y., Yamaoka, M., Shirakawa, Y., Yonemaru, K., Miyake, S., Ando, M., Ota, M., Masuda, T., Mukasa, A., Li, J., Saito, H., Hide, T., Jono, H."Wnt/β‑catenin signaling is a novel therapeutic target for tumor suppressor CYLD‑silenced glioblastoma cells". Oncology Reports 50.5 (2023): 201.
Chicago
Kanemaru, A., Ito, Y., Yamaoka, M., Shirakawa, Y., Yonemaru, K., Miyake, S., Ando, M., Ota, M., Masuda, T., Mukasa, A., Li, J., Saito, H., Hide, T., Jono, H."Wnt/β‑catenin signaling is a novel therapeutic target for tumor suppressor CYLD‑silenced glioblastoma cells". Oncology Reports 50, no. 5 (2023): 201. https://doi.org/10.3892/or.2023.8638