Pseudolaric acid B inhibits neuroglioma cell proliferation through DNA damage response

  • Authors:
    • Fengmei Song
    • Xiaoyan Yu
    • Haipeng Zhang
    • Zengyan Wang
    • Yue Wang
    • Xiangling Meng
    • Jinghua Yu
  • View Affiliations

  • Published online on: August 1, 2017     https://doi.org/10.3892/or.2017.5861
  • Pages: 2211-2218
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Abstract

Human neuroglioma is one of the most common malignant intracranial tumors in neurosurgery, and accounts for more than 50% of all brain cancer cases. Thus, a clinically effective drug with which to treat neuroglioma is urgently required. Pseudolaric acid B (PAB), a diterpene acid isolated from the root and trunk bark of Pseudolarix kaempferi Gordon (Pinaceae), was found to inhibit cell growth in a variety of cancer cell lines, but to date the effect of PAB on neuroglioma remains unclear. MTT analysis confirmed that PAB inhibited neuroglioma A172 cell growth in a time- and dose-dependent manner. In addition, PAB influenced the aggregation of tubulin in A172 cells. Meanwhile following PAB treatment, a higher percentage of cells accumulated in the G2/M phase from 12 to 48 h, while at 36 h, cell cycle slippage into the G0/G1 phase, and at 48 h, slippage into the S phase was observed using flow cytometric analysis. Corresponding protein expression was consistent with the cell cycle alteration as detected by western blotting, and it was speculated that cell cycle slippage was related to reduced effectiveness of PAB which warrants further investigation. Meanwhile PAB induced cell death by regulating p38, ERK and JNK expression and activating the DNA damage response. Therefore, PAB plays an antitumor role in A172 cells, and may be a candidate drug for neuroglioma therapy.
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October-2017
Volume 38 Issue 4

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Copy and paste a formatted citation
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Spandidos Publications style
Song F, Yu X, Zhang H, Wang Z, Wang Y, Meng X and Yu J: Pseudolaric acid B inhibits neuroglioma cell proliferation through DNA damage response. Oncol Rep 38: 2211-2218, 2017.
APA
Song, F., Yu, X., Zhang, H., Wang, Z., Wang, Y., Meng, X., & Yu, J. (2017). Pseudolaric acid B inhibits neuroglioma cell proliferation through DNA damage response. Oncology Reports, 38, 2211-2218. https://doi.org/10.3892/or.2017.5861
MLA
Song, F., Yu, X., Zhang, H., Wang, Z., Wang, Y., Meng, X., Yu, J."Pseudolaric acid B inhibits neuroglioma cell proliferation through DNA damage response". Oncology Reports 38.4 (2017): 2211-2218.
Chicago
Song, F., Yu, X., Zhang, H., Wang, Z., Wang, Y., Meng, X., Yu, J."Pseudolaric acid B inhibits neuroglioma cell proliferation through DNA damage response". Oncology Reports 38, no. 4 (2017): 2211-2218. https://doi.org/10.3892/or.2017.5861