Monoglucosyl‑rutin as a potential radioprotector in mammalian cells

Sunada,Shigeaki; Fujisawa,Hiroshi; Cartwright,Ian M.; Maeda,Junko; Brents,Colleen A.; Mizuno,Kazue; Aizawa,Yasushi; Kato,Takamitsu A.; Uesaka,Mitsuru

doi:10.3892/mmr.2014.2181

Monoglucosyl‑rutin as a potential radioprotector in mammalian cells

Authors:
- Shigeaki Sunada
- Hiroshi Fujisawa
- Ian M. Cartwright
- Junko Maeda
- Colleen A. Brents
- Kazue Mizuno
- Yasushi Aizawa
- Takamitsu A. Kato
- Mitsuru Uesaka
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Affiliations: Department of Nuclear Engineering and Management, School of Engineering, The University of Tokyo, Tokyo 113‑8656, Japan, Department of Bioengineering, School of Engineering, The University of Tokyo, Tokyo 113‑8656, Japan, Department of Environmental and Radiological Health Sciences, Colorado State University, Fort Collins, CO 80523, USA, Center for Disease Biology and Integrative Medicine, Faculty of Medicine, The University of Tokyo, Tokyo 113‑8656, Japan, Research and Development Group, Toyo Sugar Refining Co., Ltd., Yoto Bldg., Tokyo 103‑0046, Japan
Published online on: April 24, 2014 https://doi.org/10.3892/mmr.2014.2181
Pages: 10-14
Copyright: © Sunada et al. This is an open access article distributed under the terms of Creative Commons Attribution License [CC BY_NC 3.0].

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Abstract

In the present study, the role of monoglucosyl‑rutin as a potential radioprotector was investigated using mammalian cell culture models. Cell survival and DNA damage were assessed using colony formation, sister chromatid exchange and γH2AX assays. It was demonstrated that monoglucosyl‑rutin was able to increase cell survival when exposed to ionizing radiation, possibly by decreasing the amount of base damage experienced by the cell. However, the present study also demonstrated that, despite monoglucosyl‑rutin exhibiting radioprotective effects at low doses, high doses of monoglucosyl‑rutin led to a decrease in plating efficiency and an increased doubling time. This effect may be due to double‑strand breaks caused by high concentrations of monoglucosyl‑rutin.

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