Capture of dengue viruses using antibody-integrated graphite-encapsulated magnetic beads produced using gas plasma technology

Sakudo,Akikazu; Viswan,Anchu; Chou,Han; Sasaki,Tadahiro; Ikuta,Kazuyoshi; Nagatsu,Masaaki

doi:10.3892/mmr.2016.5330

Capture of dengue viruses using antibody-integrated graphite-encapsulated magnetic beads produced using gas plasma technology

Authors:
- Akikazu Sakudo
- Anchu Viswan
- Han Chou
- Tadahiro Sasaki
- Kazuyoshi Ikuta
- Masaaki Nagatsu
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Affiliations: Laboratory of Biometabolic Chemistry, School of Health Sciences, Faculty of Medicine, University of the Ryukyus, Nishihara, Okinawa 903‑0215, Japan, Department of Nanovision Technology, Graduate School of Science and Technology, Shizuoka University, Naka‑Ku, Hamamatsu 432‑8561, Japan, Department of Electrical and Electronic Engineering, Graduate School of Engineering, Shizuoka University, Naka‑Ku, Hamamatsu 432‑8561, Japan, Department of Virology, Research Institute for Microbial Diseases, Osaka University, Osaka 565‑0871, Japan
Published online on: May 24, 2016 https://doi.org/10.3892/mmr.2016.5330
Pages: 697-704
Copyright: © Sakudo et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Despite significant advances in medicine, global health is threatened by emerging infectious diseases caused by a number of viruses. Dengue virus (DENV) is a mosquito‑borne virus, which can be transmitted to humans via mosquito vectors. Previously, the Ministry of Health, Labour and Welfare in Japan reported the country's first domestically acquired case of dengue fever for almost 70 years. To address this issue, it is important to develop novel technologies for the sensitive detection of DENV. The present study reported on the development of plasma-functionalized, graphite-encapsulated magnetic nanoparticles (GrMNPs) conjugated with anti-DENV antibody for DENV capture. Radiofrequency wave‑excited inductively‑coupled Ar and ammonia gas plasmas were used to introduce amino groups onto the surface of the GrMNPs. The GrMNPs were then conjugated with an antibody against DENV, and the antibody‑integrated magnetic beads were assessed for their ability to capture DENV. Beads incubated in a cell culture medium of DENV‑infected mosquito cells were separated from the supernatant by applying a magnetic field and were then washed. The adsorption of DENV serotypes 1‑4 onto the beads was confirmed using reverse transcription‑polymerase chain reaction, which detected the presence of DENV genomic RNA on the GrMNPs. The methodology described in the present study, which employed the plasma-functionalization of GrMNPs to enable antibody‑integration, represents a significant improvement in the detection of DENV.

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