Expression analysis of progesterone‑regulated miRNAs in cells derived from human glioblastoma

Velázquez‑Vázquez,Diana Elisa; Del Moral‑Morales,Aylin; Cruz‑Burgos,Jenie   Marian; Martínez‑Martínez,Eduardo; Rodríguez‑Dorantes,Mauricio; Camacho‑Αrroyo,Ignacio

doi:10.3892/mmr.2021.12114

Expression analysis of progesterone‑regulated miRNAs in cells derived from human glioblastoma

Authors:
- Diana Elisa Velázquez‑Vázquez
- Aylin Del Moral‑Morales
- Jenie Marian Cruz‑Burgos
- Eduardo Martínez‑Martínez
- Mauricio Rodríguez‑Dorantes
- Ignacio Camacho‑Αrroyo
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Affiliations: Unidad de Investigación en Reproducción Humana, Instituto Nacional de Perinatología‑Facultad de Química, Universidad Nacional Autónoma de México, Mexico City 04510, Mexico, Oncogenomics Laboratory, The National Institute of Genomic Medicine, Mexico City 14610, Mexico, Laboratory of Cell Communication and Extracellular Vesicles, The National Institute of Genomic Medicine, Mexico City 14610, Mexico
Published online on: April 22, 2021 https://doi.org/10.3892/mmr.2021.12114
Article Number: 475
Copyright: © Velázquez‑Vázquez et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Glioblastomas (GBMs) are the most frequent and malignant type of brain tumor. It has been reported that progesterone (P4) regulates the progression of GBMs by modifying the expression of genes that promote cell proliferation, migration and invasion; however, it is not fully understood how these processes are regulated. It is possible that P4 mediates some of these effects through changes in the microRNA (miRNA) expression profile in GBM cells. The present study investigated the effects of P4 on miRNAs expression profile in U‑251MG cells derived from a human GBM. U‑251MG cells were treated for 6 h with P4, RU486 (an antagonist of the intracellular progesterone receptor), the combined treatment (P4+RU486) and cyclodextrin (vehicle) and then a miRNA microarray analysis conducted. The expression analysis revealed a set of 190 miRNAs with differential expression in the treatments of P4, RU486 and P4+RU486 in respect to the vehicle and P4 in respect to P4+RU486, of which only 16 were exclusively regulated by P4. The possible mRNA targets of the miRNAs regulated by P4 could participate in the regulation of proliferation, cell cycle progression and cell migration of GBMs. The present study provided insight for understanding epigenetic modifications regulated by sex hormones involved in GBM progression, and for identifying potential therapeutic strategies for these brain tumors.

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