miR-124 inhibits cell proliferation, migration and invasion by directly targeting SOX9 in lung adenocarcinoma

Wang,Xiaoying; Liu,Yanli; Liu,Xiaoli; Yang,Jingyan; Teng,Guoxin; Zhang,Lulu; Zhou,Chengjun

doi:10.3892/or.2016.4648

miR-124 inhibits cell proliferation, migration and invasion by directly targeting SOX9 in lung adenocarcinoma

Authors:
- Xiaoying Wang
- Yanli Liu
- Xiaoli Liu
- Jingyan Yang
- Guoxin Teng
- Lulu Zhang
- Chengjun Zhou
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Affiliations: Department of Pathology, The Second Hospital of Shandong University, Jinan, Shandong 250033, P.R. China, Provincial Key Laboratory of Radio-Oncology, Shandong Cancer Hospital and Institute, Jinan, Shandong 250117, P.R. China, Department of Clinical Laboratory, The Second Hospital of Shandong University, Jinan, Shandong 250033, P.R. China
Published online on: March 3, 2016 https://doi.org/10.3892/or.2016.4648
Pages: 3115-3121

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Abstract

Accumulating evidence indicates that dysregulation of microRNAs (miRNAs) may contribute to the initiation and progression of cancer. However, the role of miR-124 in lung adenocarcinoma (ADC) and the underlying mechanisms through which miR-124 exerts its functions are not completely understood. In the present study, we detected miR-124 and SOX9 expression in lung ADC tissues. The results showed that miR-124 was significantly downregulated in the lung ADC tissues compared with that noted in the corresponding non-cancerous lung tissues and the level of SOX9 protein was inversely associated with the expression of miR-124. The study in human lung ADC cell line A549 demonstrated that upregulation of miR-124 could inhibit cell proliferation, migration and invasion. The bioinformatic analysis showed that there was a putative miR-124 binding site in the 3' untranslated region (3'UTR) of SOX9. Using a luciferase reporter assay, we verified that SOX9 is a direct target of miR-124. Furthermore, overexpression of miR-124 repressed SOX9 expression, whereas inhibition of miR-124 increased expression of SOX9 in the A549 cells. Finally, we identified that SOX9 was a functional mediator of miR-124 in A549 cells. Taken together, our results suggest that miR-124 functions as a tumor suppressor in lung ADC by directly targeting SOX9 and it may be a promising candidate for miR‑based therapy against lung ADC.

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