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Article

miR-124 inhibits cell proliferation, migration and invasion by directly targeting SOX9 in lung adenocarcinoma

  • Authors:
    • Xiaoying Wang
    • Yanli Liu
    • Xiaoli Liu
    • Jingyan Yang
    • Guoxin Teng
    • Lulu Zhang
    • Chengjun Zhou
  • View Affiliations / Copyright

    Affiliations: Department of Pathology, The Second Hospital of Shandong University, Jinan, Shandong 250033, P.R. China, Provincial Key Laboratory of Radio-Oncology, Shandong Cancer Hospital and Institute, Jinan, Shandong 250117, P.R. China, Department of Clinical Laboratory, The Second Hospital of Shandong University, Jinan, Shandong 250033, P.R. China
  • Pages: 3115-3121
    |
    Published online on: March 3, 2016
       https://doi.org/10.3892/or.2016.4648
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Abstract

Accumulating evidence indicates that dysregulation of microRNAs (miRNAs) may contribute to the initiation and progression of cancer. However, the role of miR-124 in lung adenocarcinoma (ADC) and the underlying mechanisms through which miR-124 exerts its functions are not completely understood. In the present study, we detected miR-124 and SOX9 expression in lung ADC tissues. The results showed that miR-124 was significantly downregulated in the lung ADC tissues compared with that noted in the corresponding non-cancerous lung tissues and the level of SOX9 protein was inversely associated with the expression of miR-124. The study in human lung ADC cell line A549 demonstrated that upregulation of miR-124 could inhibit cell proliferation, migration and invasion. The bioinformatic analysis showed that there was a putative miR-124 binding site in the 3' untranslated region (3'UTR) of SOX9. Using a luciferase reporter assay, we verified that SOX9 is a direct target of miR-124. Furthermore, overexpression of miR-124 repressed SOX9 expression, whereas inhibition of miR-124 increased expression of SOX9 in the A549 cells. Finally, we identified that SOX9 was a functional mediator of miR-124 in A549 cells. Taken together, our results suggest that miR-124 functions as a tumor suppressor in lung ADC by directly targeting SOX9 and it may be a promising candidate for miR‑based therapy against lung ADC.
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Copy and paste a formatted citation
Spandidos Publications style
Wang X, Liu Y, Liu X, Yang J, Teng G, Zhang L and Zhou C: miR-124 inhibits cell proliferation, migration and invasion by directly targeting SOX9 in lung adenocarcinoma. Oncol Rep 35: 3115-3121, 2016.
APA
Wang, X., Liu, Y., Liu, X., Yang, J., Teng, G., Zhang, L., & Zhou, C. (2016). miR-124 inhibits cell proliferation, migration and invasion by directly targeting SOX9 in lung adenocarcinoma. Oncology Reports, 35, 3115-3121. https://doi.org/10.3892/or.2016.4648
MLA
Wang, X., Liu, Y., Liu, X., Yang, J., Teng, G., Zhang, L., Zhou, C."miR-124 inhibits cell proliferation, migration and invasion by directly targeting SOX9 in lung adenocarcinoma". Oncology Reports 35.5 (2016): 3115-3121.
Chicago
Wang, X., Liu, Y., Liu, X., Yang, J., Teng, G., Zhang, L., Zhou, C."miR-124 inhibits cell proliferation, migration and invasion by directly targeting SOX9 in lung adenocarcinoma". Oncology Reports 35, no. 5 (2016): 3115-3121. https://doi.org/10.3892/or.2016.4648
Copy and paste a formatted citation
x
Spandidos Publications style
Wang X, Liu Y, Liu X, Yang J, Teng G, Zhang L and Zhou C: miR-124 inhibits cell proliferation, migration and invasion by directly targeting SOX9 in lung adenocarcinoma. Oncol Rep 35: 3115-3121, 2016.
APA
Wang, X., Liu, Y., Liu, X., Yang, J., Teng, G., Zhang, L., & Zhou, C. (2016). miR-124 inhibits cell proliferation, migration and invasion by directly targeting SOX9 in lung adenocarcinoma. Oncology Reports, 35, 3115-3121. https://doi.org/10.3892/or.2016.4648
MLA
Wang, X., Liu, Y., Liu, X., Yang, J., Teng, G., Zhang, L., Zhou, C."miR-124 inhibits cell proliferation, migration and invasion by directly targeting SOX9 in lung adenocarcinoma". Oncology Reports 35.5 (2016): 3115-3121.
Chicago
Wang, X., Liu, Y., Liu, X., Yang, J., Teng, G., Zhang, L., Zhou, C."miR-124 inhibits cell proliferation, migration and invasion by directly targeting SOX9 in lung adenocarcinoma". Oncology Reports 35, no. 5 (2016): 3115-3121. https://doi.org/10.3892/or.2016.4648
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