
Prognostic significance of minichromosome maintenance mRNA expression in human lung adenocarcinoma
- Authors:
- Published online on: September 23, 2019 https://doi.org/10.3892/or.2019.7330
- Pages: 2279-2292
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Copyright: © Li et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
Abstract
Introduction
Lung cancer was the leading cause of cancer-related deaths worldwide in 2016 (1,2). Lung adenocarcinoma (LUAD), a histological type of non-small-cell lung cancer, accounts for a major proportion of this disease (3). In the last decade, the development of targeted therapy using tyrosine kinase inhibitors and crizotinib has led to improved clinical outcomes in LUAD patients with alterations in the epidermal growth factor receptor gene or fusion of echinoderm microtubule-associated protein-like 4 and anaplastic lymphoma kinase, respectively (4–7). More recently, immunotherapy has been developed and increasingly used in patients with lung cancer, including immune checkpoint inhibitors that target programmed cell death 1 ligand 1 (PD-L1)-expressing tumor cells by blocking PD-L1/PD-1 signaling (8,9). Despite recent advances in targeted therapy and immunotherapy, the prognosis of LUAD remains poor (10). It has become a research trend to explore novel molecular biomarkers or therapeutic targets in the era of precision medicine (11). In fact, databases based on large-scale, genome-wide association studies have facilitated the discovery of new biomarkers for cancer management (12).
The minichromosome maintenance (MCM) family consists of 8 highly conserved members, including MCM2-7, MCM8 and MCM10 (13). MCM2-7 form the MCM complex, a hexamer that binds to DNA and functions in the initiation of DNA replication (14,15). MCM8 is unique in that it serves as a DNA helicase during replication elongation, but not initiation (16). MCM10 helps to regulate DNA replication elongation (17). In line with their essential roles in DNA replication, MCM genes have become valuable biomarkers for cancer diagnosis and prognosis prediction (18–21). Previous studies have shown that MCM2, MCM4 and MCM7 regulate cell proliferation in non-small cell lung cancer (NSCLC) (22–24). However, the functions of other MCM family members remain unclear, and a comprehensive mRNA profiling of MCM family members in lung cancer has not been performed. In the present study, database research and bioinformatic analysis were performed to determine the prognostic significance of MCM mRNA expression in patients with lung cancer.
Materials and methods
Ethics statement
This study was performed in accordance with standard guidelines, and was approved by the Ethics Committee of the Second Affiliated Hospital, Zhejiang University School of Medicine (Zhejiang, China). The datasets were retrieved from published literature in which informed consent was obtained from patients.
Oncomine analysis
The Oncomine database (www.oncomine.org) is a bioinformatics tool for collecting, standardizing, analyzing and delivering cancer transcriptome data to the biomedical research community. It was used to compare the transcription levels of MCMs between cancer specimens and paracarcinoma tissue. In Oncomine, Student's t-test is generated for two class differential expression analyses (25). In the present study, P<0.01 and an absolute fold-change ≥1.5 were selected as the cut off values to analyze the gene expression chart of each MCM family member.
Gene expression profiling interactive analysis (GEPIA) database
GEPIA (http://gepia.cancer-pku.cn/) is a web tool that provides fast and customizable functionalities based on data from The Cancer Genome Atlas (TCGA; http://tcga-data.nci.nih.gov/tcga/) and the Genotype-Tissue Expression project (GTEx; http://www.gtexportal.org/home/index.html). Differential analysis was performed using one-way ANOVA, using disease state or tumor stage as the variable for calculating differential expression (26). In the current study, GEPIA was used to represent the differential expression of MCMs graphically between LUAD and paracarcinoma tissues and the association between the expression of MCMs and tumor stages in patients with LUAD (27).
The human protein atlas database
The Human Protein Atlas (https://www.proteinatlas.org/) is a database of immunohistochemistry (IHC)-based protein expression profiles in normal tissue, cancer and cell lines (28). IHC images of MCM protein expression in clinical specimens of patients with LUAD and paracarcinoma tissues were obtained from the Human Protein Atlas database.
Kaplan-meier plotter
The Kaplan-Meier Plotter tool (www.kmplot.com) includes survival information of 866 patients with LUAD. The prognostic value of MCM expression was assessed by overall survival (OS), progression-free survival (PFS) and post-progression survival (PPS), using the hazard ratio (HR), 95% confidence intervals (CI) and log-rank P-value. In the analysis, patient samples were split into high expression group and low expression group based on the median mRNA levels of the MCMs. The prognostic value of a gene was assessed by univariate Cox regression analysis (29). JetSet scores were used to select a single representative probe set for each gene (30). In the current study, only the probe sets with best JetSet scores for MCMs were selected to produce Kaplan-Meier plots. The one-to-one matches between MCM genes and probe sets, identified by Affymetrix IDs, were as follows: MCM2 and 272107_s_at; MCM3 and 201555_at; MCM4 and 222036_s_at; MCM5 and 216237_s_at; MCM6 and 238977_at; MCM7 and 208795_s_at; MCM8 and 224320_s_at; and MCM10 and 223570_at. The relevant concepts are defined as follows: OS, time from diagnosis to death; PFS, time from diagnosis to tumor progression; PPS, time from progression to death; HR>1, worse survival prognosis for the group with high mRNA expression; HR<1, unfavorable survival prognosis in the low mRNA expression group; 95% CI does not cross 1, mRNA expression is associated with survival rate. As not all gene expression levels were available in all patients and only the JetSet probes were included in the study, the sample sizes vary for each survival analysis.
cBioPortal for cancer genomics (cBioPortal) dataset
cBioPortal (http://cbioportal.org) is based on other authoritative databases, including the Gene Expression Omnibus (GEO; http://www.ncbi.nlm.nih.gov/geo/) and TCGA database. cBioPortal is a web resource for exploring, visualizing and analyzing multidimensional cancer genomics data. The genomic profile of each gene includes mutations, putative copy-number alterations and mRNA expression z-scores. The z score for each gene is the normalized expression of mRNA using RNA-Seq by expectation maximization count estimates method. The co-expression of each gene pair was performed by Fisher's exact test (31) and the network was constructed according to the correlation.
Cancer SEA
CancerSEA (http://biocc.hrbmu.edu.cn/CancerSEA/) is a dedicated database for comprehensively exploring distinct functional states of cancer cells at the single-cell level. The cancer-related single cell RNA-seq (scRNA-seq) datasets for human samples in CancerSEA were collected from the Sequence Read Archive (https://www.ncbi.nlm.nih.gov/sra), the GEO database and ArrayExpress (https://www.ebi.ac.uk/arrayexpress/). For each cancer-related scRNA-seq dataset, the original paper was read and the corresponding metadata was extracted, including the cancer types and sources, including patient-derived xenograft (PDX) and circulating tumor cell (CTC). Thus, CancerSEA contained the cancer single-cell functional state atlas of 41,900 cancer single cells from 25 cancer types. LUAD chips and PDX are numbered with Exp and LC-PT as the headers, respectively. For each single-cell dataset derived from PDX and CTC tumor tissue, significant correlations between gene expression and functional state activities were analyzed using Spearman's rank correlation test with false discovery rate correction for multiple comparisons (32). In the present study, CancerSEA was used for the functional analysis of MCMs.
Results
Transcriptional levels of MCMs in patients with LUAD
The MCM transcriptional levels in cancers were compared with those in paracarcinoma tissues using the Oncomine database. As presented in Fig. 1, MCMs were generally overexpressed in most tumors. In lung cancer, all MCM members were upregulated in cancer tissues, except MCM6, which was downregulated in one dataset, which may be due to the limited numbers of samples. MCM mRNA expression in LUAD and paracarcinoma tissues are summarized in Table I. The mRNA levels of all MCM members were significantly increased in LUAD tissues. MCM2 overexpression was present in 8 databases (33–39), followed by MCM4 in 7 datasets (33–37,39,40). MCM10 is the most upregulated member with a fold increase of 6.446 in the dataset from a study by Hou et al (33).
![]() | Table I.Comparison of mRNA expression of MCMs in lung adenocarcinoma and normal lung tissues from the Oncomine database. |
MCM mRNA expression is associated with pathological stages of LUAD
As with Oncomine, GEPIA analysis indicated that expression of MCM2-7, MCM8 and MCM10 was higher in LUAD than in lung tissues (Fig. 2), although statistically significant differences were observed for MCM2 (Fig. 2A), MCM4 (Fig. 2C) and MCM10 (Fig. 2H) only. The association between MCM expression and LUAD pathological stage was then investigated. As presented in Fig. 3, the mRNA levels of MCM2 (P=0.0407; Fig. 3A), MCM4 (P=0.00101; Fig. 3C), MCM6 (P=0.0096; Fig. 3E), MCM7 (P=0.00595; Fig. 3F) and MCM 10 (P=0.00598; Fig. 3H) significantly different between the tumor stages I to IV. Similar trends occurred for MCM3 (P=0.0605; Fig. 3B) and MCM5 (P=0.0957; Fig. 3D). However, there was no association between MCM8 (P=0.231; Fig. 3G) and tumor stage.
Protein expression levels of MCMs in patients with LUAD
To examine whether MCM protein was also differentially expressed in LUAD tissues, immunohistochemical staining images for the MCM proteins in LUAD and paracarcinoma tissues were obtained from the Human Protein Atlas database (Fig. 4). Consistent with RNA expression data, the results demonstrated that MCM2, MCM5, MCM6 and MCM7 protein levels were higher in LUAD tissue compared with normal tissue, whereas MCM3, MCM4 and MCM10 proteins were only slightly increased in LUAD tissue.
The prognostic significance of MCMs in patients with LUAD
Using the Kaplan-Meier plotter, the prognostic significance of the mRNA expression of MCMs in patients with LUAD was determined. Seven MCM members were significantly associated with reduced OS in patients with LUAD (Fig. 5A). Survival curves are presented in Fig. 5B-I. High expression of MCM2 (Fig. 5B; HR, 1.33; 95% CI, 1.05–1.67; P=0.018), MCM3 (Fig. 5C; HR, 2.20; 95% CI, 1.72–2.82; P=1.1×10−10), MCM4 (Fig. 5D; HR, 2.27; 95% CI, 1.78–2.90; P=1.1×10−11), MCM5 (Fig. 5E; HR, 1.34; 95% CI, 1.06–1.79; P=0.014), MCM7 (Fig. 5G; HR, 1.29; 95% CI, 1.02–1.63; P=0.031), MCM8 (Fig. 5H; HR, 1.34; 95% CI, 1.05–1.71; P=0.019) and MCM10 (Fig. 5I; HR, 1.29; 95% CI, 1.02–1.63; P=0.031) were associated with worse OS, while MCM6 was not associated with altered OS (Fig. 5F; HR, 1.08; 95% CI, 0.85–1.37; P=0.530).
Similarly, high expression of MCM2 (Fig. 6B; HR, 1.62; 95% CI, 1.18–2.22; P=0.003), MCM3 (Fig. 6C; HR, 1.39; 95% CI, 1.02–1.90; P=0.037), MCM4 (Fig. 6D; HR, 2.38; 95% CI, 1.72–3.31; P=8.3×10−8), MCM5 (Fig. 6E; HR, 1.42; 95% CI, 1.04–1.95; P=0.027), MCM8 (Fig. 6H; HR, 1.44; 95% CI, 1.04–1.99; P=0.027) and MCM10 (Fig. 6I; HR, 1.94; 95% CI, 1.40–2.71; P=6.5×10−5) was significantly associated with reduced PFS. Additionally, high MCM4 mRNA expression also indicated adverse PPS (Fig. 6D; HR, 1.62; 95% CI, 1.01–2.59; P=0.043). Other MCMs were not associated with PFS or PPS (Figs. 6 and 7).
MCM expression changes in LUAD and the network within each MCM or with other genes
With the cBioPortal online tool, the alterations, correlations and networks of MCMs in LUAD were analyzed. Of the LUAD samples, 39% had altered MCMs, and the most common genetic change was gene amplification (Fig. 8A). The distribution of genetic alterations for individual MCMs indicated that nearly 15% of LUAD cases had MCM4 amplification (Fig. 8B). Significant and positive correlations were observed between the MCMs (Fig. 8C). Among them, MCM2 and MCM6 had the highest positive correlation with a Spearman's correlation coefficient of 0.848. MCM10 exhibited the tightest association with all other MCMs, with a median Spearman's correlation coefficient of 0.771. Next, the network for MCMs and the genes frequently altered with MCMs was constructed (Fig. 8D). The majority of the genes frequently altered with MCMs were cell cycle-related or involved in DNA damage/repair, such as ATM serine/threonine kinase, RAD1 checkpoint DNA exonuclease, cyclin dependent kinase 1, cyclin A1 and replication factor C subunit 5, suggesting that the MCM family is critical for maintenance of genome integrity.
The functions of MCMs in single LUAD cell
Heterogeneity between cancer cells poses a major challenge for cancer diagnosis and treatment. Single-cell sequencing technology provides an unprecedented opportunity to accurately decipher the functional states of cancer cells at a single-cell resolution.
Using CancerSEA, the functions of MCMs in single LUAD cells were explored. MCM function was found to be mainly related to cell cycle, DNA damage or DNA repair (Fig. 9A). Kim (Exp0066) showed high expression of the MCM family was positively correlated with DNA repair, cell cycle, DNA damage and proliferation (Spearman's coefficients, 0.60, 0.56, 0.50 and 0.32, respectively; P<0.001) (41). Correlation analysis also revealed a negative correlation of MCM expression and quiescence, inflammation, hypoxia and differentiation (Spearman's coefficient, −0.41, −0.32, −0.31 and −0.31, respectively; P<0.001) (Fig. 9B). Similar results were observed in patient-derived xenograft (LC-PT-45) (41) (Fig. 9C).
Discussion
Uncontrolled cancer cell proliferation is usually accompanied by dysregulated DNA replication, and chemotherapeutic agents targeting replication machinery have been widely used for cancer treatment (42). MCMs, essential molecules in the initiation and elongation of DNA replication, are considered to be useful indicators of cell proliferation, and MCM alterations are more frequent in neoplastic cells than in non-neoplastic cells (43). Moreover, MCM proteins are expressed in rapidly dividing cells, but not in quiescent, aging or differentiated cells (44). Therefore, MCMs may have potential clinical application as markers for cancer screening. However, a comprehensive bioinformatics analysis of MCMs in LUAD has yet to be performed. To the best of our knowledge, this is the first study to explore the mRNA expression levels of the MCM family and their prognostic relevance for predicting OS, PFS and PPS in LUAD. Our findings highlight potential roles for MCMs in diagnosis and risk stratification in patients with LUAD.
MCM2 is the most studied MCM member that is upregulated in LUAD, and MCM2 dysregulation is associated with cell proliferation, cell cycle progression and migration (45). A significant correlation between MCM2 mRNA expression and LUAD stages was previously observed, and MCM2 was demonstrated to be the therapeutic target of lovastatin for NSCLC treatment (46). Additionally, Veena et al (47) reported that the MCM2 was present in lung tissues and in sputum, which is more accessible for MCM2 detection. However, the association between MCM2 expression and treatment outcomes of patients with NSCLC remains controversial (44,48–50). In the present study, analysis using the Oncomine and GEPIA bioinformatics tools revealed that MCM2 expression was higher in LUAD tissues compared with that in normal tissues, and this finding was consistent with immunohistochemical staining for MCM2 in the Human Protein Atlas. Using the Kaplan-Meier Plotter, elevated MCM2 mRNA was found to be significantly associated with reduced OS and PFS in patients with LUAD.
MCM3 had been found to be differentially expressed in LUAD and adjacent normal tissue samples (51,52); however, the role of MCM3 in NSCLC was unclear. Zhang et al (53) found that the PI3K inhibitor, LY294002, inhibited MCM3 expression in NSCLC cells, indicating that MCM3 is involved in the PI3K-Akt pathway. In the present study, MCM3 upregulation was also observed in LUAD tissues, and high MCM3 expression was associated with poor OS and PFS, but not PPS in patients with LUAD.
MCM4 overexpression is an oncogenic event in LUAD (54). A study by Kikuchi et al (55) showed that high MCM4 expression was associated with clinicopathological features of LUAD, but was not associated with survival. However, in the present study, high expression of MCM4 was associated with worse OS, PFS and PPS.
MCM5 is upregulated in lung squamous cell carcinoma, and patients with high MCM5 expression have reduced OS (43). In the current study, MCM5 was also found to be elevated in LUAD and was associated with adverse OS and PFS.
MCM6, together with Ki-67 and HuR, an RNA binding protein, were associated with poor prognosis in NSCLC (44,56). However, no association was found between MCM6 expression and patient outcome in the current study.
MCM7 is involved in proliferation and signal transduction of the receptor of activated protein C kinase 1/Akt pathway (57). Moreover, pre-clinical research based on NSCLC cell lines showed that MCM7 and its targets, a cluster of miR-25, miR-93 and miR-106b, elicited oncogenic activity in lung cancer (58). MCM7 expression was also associated with worse prognosis in patients with NSCLC (59,60). In the present study, high MCM7 expression was suggested to be a predictor for poor OS and PFS.
Little is known about the role of MCM8 and MCM10 in lung cancer. MCM8 is expressed in tissue with a high percentage of proliferating cells, such as lung and liver (13,16). MCM10 promotes cell proliferation (17), is associated with poor prognosis and is considered to be a potential therapeutic target in breast and prostate cancer (61). In the current study, MCM8 mRNA, but not protein expression was found to be is higher in tumor compared with normal tissues. Similarly, MCM10 was also found to be overexpressed in LUAD. However, neither MCM8 nor MCM10 were associated with the OS of patients with LUAD.
MCMs have frequently been compared with Ki-67, a well-established marker of cell proliferation (62). In adrenocortical cancer, MCM3 and MCM7 share a similar staining pattern with Ki-67 in benign and malignant tissues (63). MCM4 and MCM7 are more sensitive markers than Ki-67 for the detection of esophageal cancer (64). Furthermore, MCM5 and MCM2, but not Ki-67, have been associated with poor prognosis in gastric cancer and NSCLC (65,66). As a marker of G1-phase arrest in mantle cell lymphoma, MCM6 is superior to other clinical parameters, including Ki-67 (48). MCM2 expression is higher than Ki-67 in megakaryocytes, suggesting that MCM2 may be a more sensitive marker for some hematological diseases (67). In tissue sections, MCM proteins are more highly expressed than Ki-67 in quiescent cells (68,69). Taken together, these results suggest that the MCM family may be better proliferation markers than Ki-67 for cancer diagnosis and risk stratification.
In the present study, the expression and prognostic significance of MCMs in LUAD was analyzed. MCM2-6 were found to be upregulated and associated with LUAD prognosis. Notably, high expression of MCM2-5 was associated with reduced OS and PFS. MCM7 expression was also associated with poor OS, and MCM4 predicted worse PPS. As MCM expression was found to be consistent at the mRNA and protein level, analysis of mRNA in patient samples may be preferred as it is easier to analyze. Thus, transcriptional detection of MCM members may provide robust biomarkers to improve patient survival and prognosis prediction in LUAD. As all data and results in this study are based on bioinformatic analysis, further studies are required to determine the role of MCMs in vitro and in vivo.
Acknowledgments
Not applicable.
Funding
This study was supported by the National Natural Science Foundation of China (grant no. 81572920), the Natural Science Foundation of Zhejiang Province of China (grant no. LY15H160038) and the National Basic Research Program of China (grant no. 2013CB911303).
Availability of data and materials
The datasets analyzed during the current study are publicly available from the following online databases: Oncomine database (www.oncomine.org); GEPIA database (http://gepia.cancer-pku.cn/); Kaplan-Meier Plotter (www.kmplot.com); The Human Protein Atlas (https://www.proteinatlas.org/); cBioPortal (http://cbioportal.org); GEO (http://www.ncbi.nlm.nih.gov/geo/); TCGA (https://tcga-data.nci.nih.gov/tcga/); CancerSEA (http://biocc.hrbmu.edu.cn/CancerSEA/).
Authors' contributions
SL and YX designed the study. SL, ZJ and YL acquired the data, performed data analysis and interpretation. ZJ and YL drafted the manuscript, and YX and SL critically revised it for important intellectual content. All authors gave final approval of the version to be published, and agreed to be accountable for all aspects of the work.
Ethics approval and consent to participate
This study was performed in accordance with standard guidelines and was approved by the Ethics Committee of the Second Affiliated Hospital, Zhejiang University School of Medicine.
Patient consent for publication
Not applicable.
Competing interests
The authors declare that they have no competing interests.
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