Role of glucose metabolism in the differential antileukemic effect of melatonin on wild‑type and FLT3‑ITD mutant cells

  • Authors:
    • Noelia Puente‑Moncada
    • Maria Turos‑Cabal
    • Ana M. Sánchez‑Sánchez
    • Isaac Antolín
    • Federico Herrera
    • Jezabel Rodriguez‑Blanco
    • Cristina Duarte‑Olivenza
    • Carmen Rodriguez
    • Vanesa Martín
  • View Affiliations

  • Published online on: April 15, 2020     https://doi.org/10.3892/or.2020.7584
  • Pages: 293-302
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

The FMS‑like tyrosine kinase 3 internal tandem duplication (FLT3‑ITD) mutation represents the most frequent genetic alteration in acute myeloid leukemia (AML) and is associated with poor prognosis. The mutation promotes cancer cell survival and proliferation, and shifts their glucose metabolism towards aerobic glycolysis, a frequent alteration in cancer. In the present study, the impact of melatonin on the viability of AML cell lines with (MV‑4‑11 and MOLM‑13) or without the FLT3‑ITD mutation (OCI‑AML3 and U‑937) was evaluated. Melatonin induces cell death in AML cells carrying the FLT3‑ITD mutation, but only inhibits the proliferation of AML cells without this mutation. Consistently, melatonin decreases tumor growth and increases animal survival in a xenograft model of FLT3‑ITD AML. Toxicity is related to a decrease in glucose uptake, lactate dehydrogenase activity, lactate production and hypoxia‑inducible factor‑1α activation. Melatonin also regulates the expression of glucose metabolism‑related genes, impairing the balance between anaplerosis and cataplerosis, through the upregulation of the expression of phosphoenolpyruvate carboxykinase 2 (PCK2). Collectively, the present findings highlight the regulation of glucose metabolism, currently considered a possible therapeutic target in cancer, as a key event in melatonin‑induced cytotoxicity, suggesting its potential as a therapeutic tool for the treatment of patients with AML, particularly those carrying the FLT3‑ITD mutation that results in low basal expression levels of PCK2.
View Figures
View References

Related Articles

Journal Cover

July 2020
Volume 44 Issue 1

Print ISSN: 1021-335X
Online ISSN:1791-2431

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
APA
Puente‑Moncada, N., Turos‑Cabal, M., Sánchez‑Sánchez, A.M., Antolín, I., Herrera, F., Rodriguez‑Blanco, J. ... Martín, V. (2020). Role of glucose metabolism in the differential antileukemic effect of melatonin on wild‑type and FLT3‑ITD mutant cells. Oncology Reports, 44, 293-302. https://doi.org/10.3892/or.2020.7584
MLA
Puente‑Moncada, N., Turos‑Cabal, M., Sánchez‑Sánchez, A. M., Antolín, I., Herrera, F., Rodriguez‑Blanco, J., Duarte‑Olivenza, C., Rodriguez, C., Martín, V."Role of glucose metabolism in the differential antileukemic effect of melatonin on wild‑type and FLT3‑ITD mutant cells". Oncology Reports 44.1 (2020): 293-302.
Chicago
Puente‑Moncada, N., Turos‑Cabal, M., Sánchez‑Sánchez, A. M., Antolín, I., Herrera, F., Rodriguez‑Blanco, J., Duarte‑Olivenza, C., Rodriguez, C., Martín, V."Role of glucose metabolism in the differential antileukemic effect of melatonin on wild‑type and FLT3‑ITD mutant cells". Oncology Reports 44, no. 1 (2020): 293-302. https://doi.org/10.3892/or.2020.7584