Open Access

Cep63 knockout inhibits the malignant phenotypes of papillary thyroid cancer cell line TPC‑1

  • Authors:
    • Chenguang Liu
    • Fangqin Yu
    • Runsheng Ma
    • Lele Zhang
    • Gongbo Du
    • Dongpeng Niu
    • Detao Yin
  • View Affiliations

  • Published online on: July 21, 2021     https://doi.org/10.3892/or.2021.8150
  • Article Number: 199
  • Copyright: © Liu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The present study was designed to observe the expression of the centrosomal protein 63 in papillary thyroid cancer (PTC) tissues and cells and to explore the clinical significance of Cep63 expression in PTC. Primary PTC tissues and matched normal thyroid tissues were collected, and the Cep63 expression level was determined by reverse transcription‑quantitative PCR and western blotting. A stable Cep63‑knockout cell line was constructed to assess the proliferation, invasion, migration and apoptosis abilities in vitro. A subcutaneous tumorigenesis model was established in nude mice to evaluate the effect of Cep63 on tumor growth and proliferation in vivo. Western blotting was used to explore the relevant signaling pathways. The results revealed that the expression level of Cep63 in PTC tissues was significantly increased. The proliferation, invasion and migration abilities of TPC‑1 cells were decreased after Cep63 knockout, and silencing of Cep63 resulted in TPC‑1 cell cycle arrest in the S phase. Mechanistically, Cep63 knockout inhibited the activation of the Janus kinase/signal transducer and activator of transcription 3 signaling pathway. In conclusion, Cep63 knockout significantly inhibited biological functions of TPC‑1 cells in vitro and in vivo, indicating that Cep63 may be an important oncogene of PTC.

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September-2021
Volume 46 Issue 3

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Copy and paste a formatted citation
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Spandidos Publications style
Liu C, Yu F, Ma R, Zhang L, Du G, Niu D and Yin D: Cep63 knockout inhibits the malignant phenotypes of papillary thyroid cancer cell line TPC‑1. Oncol Rep 46: 199, 2021
APA
Liu, C., Yu, F., Ma, R., Zhang, L., Du, G., Niu, D., & Yin, D. (2021). Cep63 knockout inhibits the malignant phenotypes of papillary thyroid cancer cell line TPC‑1. Oncology Reports, 46, 199. https://doi.org/10.3892/or.2021.8150
MLA
Liu, C., Yu, F., Ma, R., Zhang, L., Du, G., Niu, D., Yin, D."Cep63 knockout inhibits the malignant phenotypes of papillary thyroid cancer cell line TPC‑1". Oncology Reports 46.3 (2021): 199.
Chicago
Liu, C., Yu, F., Ma, R., Zhang, L., Du, G., Niu, D., Yin, D."Cep63 knockout inhibits the malignant phenotypes of papillary thyroid cancer cell line TPC‑1". Oncology Reports 46, no. 3 (2021): 199. https://doi.org/10.3892/or.2021.8150